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alphadigidoc pro imaging system  (Alpha Innotech)
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digital microscope dino-lite edge am4115zt  (AnMo Electronics)
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image analysis software  (Carl Zeiss)
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Image Search Results


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Journal: Molecular cell

Article Title: Cancer cells employ nuclear caspase-8 to overcome the p53-dependent G2/M checkpoint through cleavage of USP28

doi: 10.1016/j.molcel.2019.12.023

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: After blocking with 5% FCS in PBS (++) for 30 min at RT, primary antibodies against caspase-8 (#PA5-21336, Thermo Scientific), cleaved caspase-8 (#sc-166320; Santa Cruz), and myc-tag (#ab18185, Abcam), respectively, were incubated for at RT 2 h. After washing, Alexa-488 or −546 coupled isotype-specific secondary antibodies (#A11034, #A11030, Life Technologies) were incubated at RT for 1 h, coverslips washed, and mounted in Mowiol (#D2522, Sigma).

Techniques: Western Blot, Immunocytochemistry, Immunohistochemistry, Recombinant, Protease Inhibitor, Enzyme-linked Immunosorbent Assay, Extraction, Imaging, Software